Table 1.

Immunohistochemical analysis of the Jagged ligands, Notch activation and angiogenesis in MM patients’ bone marrow biopsies (BMBs). The degree of malignant plasma cell infiltration in the BM is reported along with the antigen immunoreactivity for monoclonal or Ig light chains. Additionally, the percentage of immunoreactive cells out of the total cells for Jagged1 and 2, HES6 and VEGF-A is reported both for MM (M) and non-MM (NM) cells, along with the microvessel density (MVD) evaluated as the average number of cell determinant 34+ (CD34+) vessels per field.

PATIENT N.	INFILTRATION DEGREE	LIGHT CHAIN	JAGGED1 M	JAGGED1 NM	JAGGED2 M	JAGGED2 NM	HES6 M	HES6 NM	VEGF-A M	VEGF-A NM	MVD
1	I	K	50	5	20	15	5	5	0	30	13.0
2	I	L	30	5	35	40	15	5	0	40	30.3
3	I	L	30	9	20	25	9	5	0	15	32.0
4	I	L	40	9	20	20	20	9	0	35	16.0
5	I	L	15	9	15	20	5	9	0	35	29.3
6	I	L	20	5	65	35	35	5	0	31	24.7
7	I	K	25	5	70	40	40	5	0	10	19.0
8	I	K	35	9	65	80	40	9	0	45	55.7
9	I	L	30	9	65	60	25	5	0	20	37.3
10	I	L	25	9	55	30	35	5	0	25	25.3
11	II	L	25	15	65	40	40	5	20	70	60.0
12	II	K	30	15	70	45	55	15	15	50	59.7
13	II	L	35	20	70	70	15	15	35	65	38.7
14	II	K	40	35	65	75	20	5	45	80	39.7
15	II	K	40	25	70	50	45	20	20	75	57.7
16	II	K	40	25	40	55	15	15	25	55	32.7
17	II	K	85	15	35	80	50	10	50	70	74.3
18	II	K	60	40	70	75	60	10	60	70	53.7
19	II	K	80	30	75	55	70	5	30	65	71.3
20	II	L	55	25	65	80	65	15	50	65	62.7
21	II	K	60	50	70	85	70	20	40	70	67.3
22	III	K	30	25	85	85	55	20	70	80	70.3
23	III	K	80	55	75	90	60	15	75	85	75.0
24	III	L	65	25	60	80	70	15	50	85	68.3
25	III	L	80	30	90	85	80	10	55	85	79.0
26	III	K	70	30	95	90	85	20	80	80	86.0
27	III	K	75	25	75	80	45	20	85	70	66.7
28	III	K	55	30	80	80	60	25	50	85	70.0
29	III	K	80	50	80	90	80	35	40	90	78.0
30	III	K	80	30	80	95	N.A.	25	45	80	79.0
31	III	K	75	25	85	95	80	20	40	80	89.0
32	III	L	85	55	90	90	85	40	80	65	86.3
33	III	K	70	45	80	85	80	35	40	80	89.7
34	III	K	80	30	90	85	90	20	50	70	74.0