Table 3.

Antibodies and experimental conditions used for the IHC.

Antigen	Clone	Source	Dilution/Time	Unmasking
Kappa light chain	-	Agilent	1:10; 1 h	FLEX TRS Low pH
Lambda light chain	-	Agilent	1:10; 1 h	FLEX TRS Low pH
Jagged 1	AF1277 Goat	R&D syst.	1:100; 1 h	EDTA
Jagged 2	4F10 Mouse	Santa Cruz	1:200; o.n.	Citrate
HES6	Polyclonal Rabbit	Abcam	1:300; o.n.	Citrate
VEGF-A	A-20 Polyclonal Rabbit	Santa Cruz	1:800; 1 h	Citrate
CD34	QBEnd 10 Monoclonal Mouse	Agilent	Ready-to-Use	FLEX TRS Low pH

Statistical analysis and percentage variation among the HMCL experimental conditions.