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. 2020 Sep 1;10(3):109. doi: 10.3390/jpm10030109

Figure 1.

Figure 1

Survival and migration were decreased in response to Sestrin2 knockdown in A549 lung cancer cells. (A) Expression of Sestrin2 in shSESN2-1, shSESN2-2, and scramble A549 cells as measured by RT-PCR followed by subsequent agarose gel analysis. Band density was measured with ImageJ and is plotted as the value relative to scramble control. (B) Survival of scramble, shSESN2-1, and shSESN2-2 cells was measured using trypan blue staining. The number of cells was counted using a hemocytometer. (C) The wound healing assay revealed cell movement capacity. Cells were observed at the indicated time, and closure percentage is plotted. The photo was taken under an inverted light microscope and closure percentage was measured using ImageJ. (D) The mRNA expression of epithelial–mesenchymal transition markers (Vimentin, Snail, ZEB1) was downregulated in Sestrin2 knockdown cells compared to that in scramble cells. Band density was measured using ImageJ and is plotted on the right (** p < 0.01; *** p < 0.005; **** p < 0.0001).