Figure 1.

Effects of caspase inhibitor on cell viability and enhanced green fluorescence protein (EGFP) expression in Jurkat cells. Cells were treated with z-vad-fmk at different concentrations for 24 h post pulsing. (A) Cell viability is defined as the ratio of propidium iodide (PI) negative cells between the experimental and the non-pulsed control groups. The electrotransfer efficiency was characterized by three parameters: (B) Electrotransfer effectiveness (eTE), the percent of PI-cells that expressed the transgene; (C) expression level, the geometry mean of transgene expression level among the cells that were PI- and expressed the gene; (D) effective expression level, the product of eTE, expression level, and cell viability, which was a measure of the average gene expression level among all cells in a group. Pulsing condition: 650 V/0.2 cm, 400 µs, 1 pulse. Error bars, SEM; * p < 0.05, Student’s t-test. N (biological replicate number) = 6.