Figure 2.

Effects of caspase inhibitor on cell viability and EGFP expression in Jurkat cells under two different experimental conditions. One (i.e., no incubation) was the same as that in Figure 1; the other was similar to the first one except that the cells were incubated in the pulsing buffer for 5 min before being treated with z-vad-fmk at different concentrations for 24 h. (A) Cell viability; (B) eTE; (C) expression level; (D) effective expression level. Pulsing condition: 650 V/0.2 cm, 400 µs, 1 pulse. Error bars, SEM; * p < 0.05, Student’s t-test. N = 6.