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Percent of apoptotic cells after treatment with pharmacological inhibitor. Cells were treated with z-vad-fmk at different concentrations for 8 h post pulsing. (A) Western blot analysis at 8 h post pulsing. (B) Percent of apoptotic cells (Annexin V+). N = 6. NP, non-pulsed. Two pulsing conditions were tested. Condition 1: 650 V/0.2 cm, 400 µs, 1 pulse; Condition 2: 550 V/0.2 cm, 300 µs, 2 pulses, 10 Hz. Error bars, SEM.
