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. 2020 Sep 11;12(9):2603. doi: 10.3390/cancers12092603

Figure 7.

Figure 7

Effects of apoptosis inhibition on cell viability and indel frequency in Jurkat cells. The gene-editing was achieved through electrotransfer of a ribonucleoprotein (RNP) targeting the T cell receptor-α constant (TRAC) gene. (A) Inference of CRISPR Edits (ICE) analysis, showing the outcome of TRAC editing in Jurkat cells; (B) cell viability measured at 24 h; (C) indel within the TRAC gene determined at 48 h post electrotransfer using TIDE analysis. Pulsing condition: 650 V/0.2 cm, 300 µs, 2 pulses, 10 Hz. ns, non-significant. Error bars, SEM; * p < 0.05, Student’s t-test, N = 6.