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Synergistic effect of mEHT and heat shock inhibitors. 4T1 cells pre-treated with heat shock response inhibitors, Quercetin or KRIBB11, or vehiculum (DMSO) for 1 h before mEHT (42 °C, 30 min) treatment. (A) Resazurin viability assay, 24 h post-mEHT, viability expressed as percent of control (37 °C, vehiculum only). (B) Hsp70 mRNA 2 h post- mEHT, normalized to 18S rRNA. Two-way ANOVA, Mean ± SEM, n = 5–12/group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
