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. 2020 Sep 21;12(9):2696. doi: 10.3390/cancers12092696

Figure 1.

Figure 1

Cell viability and proliferation of AML cell lines cultured in the presence of Wnt and GSK-3 inhibitors. AML cells lines were stained with CFSE and cultured in the presence of either Wnt modulators, including PNU-74654 (15 µM), IWP-2 (15 µM) Niclosamide (1 µM), or GSK-3 inhibitors, including LiCl (10 mM), AR-A014418 (15 µM). After 4 days, cells were stained with TOPRO-3 to exclude dead cells. (A) Viable cells (TOPRO-3 negative cells) and CFSE-stained cells were quantified by FACS analysis. (B) Relative cell proliferation was expressed as the percentage of CFSE median fluorescence of treated cells compared to the cells treated with DMSO. Data are reported as mean ± SEM of 5 independent experiments. A Mann–Whitney test was used to analyze the differences between means. * p < 0.05, ** p < 0.01, *** p < 0.001. Dot line: control.