Table 1.
Flow cytometric analysis of Wnt/β-catenin and GSK-3 molecules in AML cell lines. HL-60, THP1 and U937 were probed with specific primary antibodies and labelled with Alexa 488-conjugated secondary antibodies. Data are expressed as relative median of fluorescence intensity and reported as mean ± SEM of 6 independent experiments.
| Relative Expression of Wnt Molecules in AML Cell Lines | HL-60 | THP1 | U937 |
|---|---|---|---|
| Relative median of fluorescence intensity (rMFI) ± SEM | |||
| Total β-catenin | 2.466 ± 0.238 | 6.765 ± 1.508 | 2.781 ± 0.288 |
| Non-phospho-β-catenin | 1.676 ± 0.058 | 2.360 ± 0.209 | 1.442 ± 0.0677 |
| Ser675-phospho-β-catenin | 3.471 ± 0.202 | 7.847 ± 1.443 | 3.398 ± 0.566 |
| Ser33/37/Thr41-phospho-β-catenin | 2.135 ± 0.119 | 3.013 ± 0.395 | 2.232 ± 0.21 |
| GSK-3α | 2.275 ± 0.161 | 2.654 ± 0.298 | 2.194 ± 0.18 |
| pGSK-3α (Ser21) | 9.355 ± 1.641 | 1.640 ± 2.678 | 7.901 ± 1.643 |
| GSK-3β | 2.217 ± 0.139 | 2.456 ± 0.293 | 1.713 ± 0.064 |
| GSK-3β (Ser 9) | 4.600 ± 0.416 | 9.401 ± 3.046 | 3.935 ± 0.643 |