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. 2020 Sep 7;9(9):276. doi: 10.3390/biology9090276

Figure 2.

Figure 2

Box-and-whisker plot (horizontal line: median; box: 25/75 percentile; whisker: 10/90 percentile) showing the differences in cytokine concentrations measured in pig seminal plasma samples (SP) stored at 5 °C, −20 °C and −80 °C for 72 h with respect to those measured in fresh samples (baseline samples, point 0 in Y axis). Below the X-axis is the concordance correlation coefficient (ρc) and 95% confidence interval (CI), showing agreement between cytokine concentrations of baseline and experimental SP samples. Cytokines: Granulocyte macrophage colony-stimulating factor (GM-CSF), interferon-gamma (IFNγ), interleukin (IL)-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and tumor necrosis factor-α (TNFα). Asterisks indicate statistical differences with baseline data. *** p < 0.001; ** p < 0.01; * p < 0.05.