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. 2020 Aug 21;8(3):465. doi: 10.3390/vaccines8030465

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Generation and purification. A schematic diagram of H6 virus-like particle (VLP) construction and expression. (A) A schematic diagram of H6 VLP construction and expression. The recombinant plasmid carrying the hemagglutinin (HA) and matrix protein 1 (M1) genes derived from the H6 avian influenza virus (AIV) was transposed onto the bacmid DNA. (B) VLPs that assembled HA and M1 proteins were harvested in the rBac-H6M1-infected Sf21 culture supernatant. (C) The H6 VLPs were purified through sucrose density gradient centrifugation. (D) The hemagglutination activity of purified VLPs was verified with the HA test. (E) The expression of the HA and M1 proteins on the VLPs was analyzed using SDS-PAGE and validated by Western blot using the antiserum directed against H6 AIVs.