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. 2020 Aug 21;8(3):465. doi: 10.3390/vaccines8030465

Figure 3.

Figure 3

The immunogenicity of H6 VLPs with ISA 71 VG in a chicken model. (A) Specific-pathogen-free (SPF) chickens were prime-boost vaccinated on Days 0 and 21 with PBS, VLPs alone, or VLPs with commercial adjuvant ISA 71 VG, and blood samples were collected. H6N1 AIV-specific IgG titers were analyzed by ELISA on day 21. (B) H6N1 AIV-specific IgG titers were analyzed by ELISA on day 56. (C) H6N1 AIV-specific HI titers were analyzed by a HI test. Error bars represent the means ± S.D. The horizontal dotted lines mark the detection limit for the antibody titer. Serum IgG titers among groups were compared using one-way ANOVA followed by Dunnett’s multiple-comparison test. The HI titers among vaccination groups and the control group were compared using two-way ANOVA followed by Tukey’s multiple-comparison test, and the differences between the VLP alone and ISA 71 VG-adjuvanted groups are shown (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).