TABLE 2.
Kinetic rates and dissociation constants of CH103 lineage MAbs and VRC01 bnAb to CH505TFv4.1 ch.SOSIP and CH505TF.D902 gp150 trimers with and without BMS-529a
| Group | Lineage | ka (×104 ms−1) | kd (×10−4 s−1) | KD (nM) |
|---|---|---|---|---|
| CH505TF.D902 gp150 | CH103 UCA | 2.68 | 177.5 | 661.8 |
| CH103 IA4 | 4.31 | 21.72 | 50.4 | |
| CH106b | 4.23 | 61.26 | 145.0 | |
| 0.58 | <0.1 | <1.0 | ||
| VRC01 | 3.16 | 7.08 | 22.4 | |
| CH505TFv4.1 ch.SOSIP | CH103 UCA | 2.86 | 128.6 | 449.3 |
| CH103 IA4 | 6.42 | 10.33 | 16.1 | |
| CH106 | 1.99 | 0.88 | 4.4 | |
| VRC01 | 3.27 | 9.24 | 28.2 | |
| CH505TF.D902 gp150+BMS | CH103 UCA | 1.18 | 227.6 | 1934 |
| CH103 IA4 | 3.65 | 203.3 | 557.4 | |
| CH106b | 1.47 | 56.26 | 382.0 | |
| 0.40 | 1.13 | 28.3 | ||
| VRC01 | 3.42 | 11.81 | 34.5 | |
| CH505TF v4.1 ch.SOSIP+BMS | CH103 UCA | 2.87 | 605.9 | 2110 |
| CH103 IA4 | 3.30 | 184.4 | 559.1 | |
| CH106 | 1.18 | 0.45 | 3.8 | |
| VRC01 | 1.84 | 3.60 | 19.6 |
a
Data are representative of two independent experiments.
b
Data with a biphasic curve, and both fast and slow phases are reported.