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Infection with ASFV does not induce miRNA polyadenylation or decay. (a) Northern blot of RNA extracted from Vero cells and porcine alveolar macrophages (PAMs) that were infected with either ASFV or VACV. The blot was probed for miR-27b-3p and 5s and imaged on a Phosphorimager. Arrow, mature miRNA; asterisk, polyadenylated miRNA. (b) Phosphorimager quantification of miR-27b-3p expression normalized to 5S rRNA. The image and quantification is a representation of two biological repeats.
