FIG 2.

ASFV does not induce widespread polyadenylation or decay of cellular miRNAs. (a and b) The proportion of sequencing reads that mapped to known host miRNAs that were PAM infected or mock infected with ASFV (a) and in HeLa cells infected or mock infected with VACV at early and late time points (b). (c and d) The proportion of 3′ modified miRNA that contained at least 1 nontemplated nucleotide after position 19 as a proportion of total sequencing reads, in ASFV mock-infected and infected PAMs (c) and VACV mock-infected and infected HeLa cells (d). (e) Proportion of miRNA containing 3 or more 3′ nontemplated adenosine residues after nucleotide position 19 in ASFV mock-infected and infected PAMs. Data represent the means from three biological repeats, and error bars represent standard errors of the means (SEM). Data were statistically analyzed using Student’s t test. ns, P > 0.05; ***, P < 0.001; ****, P < 0.0001. Data from panels b and d were taken from supporting information from reference 18 and represent means from three biological replicates.