TABLE 8.
List of primers used in this study
| Primer | Sequence (5′–3′)a | Description |
|---|---|---|
| NSP2-OTU-F | CGCGTCTCGAATTCAAGAGAGCAAGGAAAACA | Cloning of NSP2 OTU |
| NSP2-OTU-R | CGCGTCTCCTCGAGAACGGTTGGAGTCGTCGG | Cloning of NSP2 OTU |
| NSP1β-1-nanoluc-F | GGCGGTGGAGGCAGTGAATTCTTCGAGCCCGTGCCCAAG | Cloning of NSP1β-1–NanoLuc |
| NSP1β-1-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTAGTGGGGGGGGAAG | Cloning of NSP1β-1–NanoLuc |
| NSP1β-2-nanoluc-F | GGCGGTGGAGGCAGTGAATTCCAGGTGAACGGGCTGAGA | Cloning of NSP1β-2–NanoLuc |
| NSP1β-2-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTACTGGATCACGATG | Cloning of NSP1β-2–NanoLuc |
| NSP2-1-nanoluc-F | GGCGGTGGAGGCAGTGAATTCAGAAGCGGCGCCACCACC | Cloning of NSP2-1–NanoLuc |
| NSP2-1-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTAGGCGCTACTAGCG | Cloning of NSP2-1–NanoLuc |
| NSP3-nanoluc-F | GGCGGTGGAGGCAGTGAATTCCTGACCATCCTGTGGCTG | Cloning of NSP3–NanoLuc |
| NSP3-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTAGTTCACGGAGATC | Cloning of NSP3–NanoLuc |
| NSP7α-nanoluc-F | GGCGGTGGAGGCAGTGAATTCATCGAGCTGGCCCAGCTG | Cloning of NSP7α–NanoLuc |
| NSP7α-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTAGGTTCCTCTCACC | Cloning of NSP7α–NanoLuc |
| NSP10-nanoluc-F | GGCGGTGGAGGCAGTGAATTCCACGTGGAGCAGGGGCTG | Cloning of NSP10–NanoLuc |
| NSP10-nanoluc-R | CTGCACCTGAGGAGTGCGGCCGCTTACAGCCCCCTTCTG | Cloning of NSP10–NanoLuc |
| Tubulin-F | GGCTGTCTGCGATATCCCTC | For qPCR detection |
| Tubulin-R | TGCTCTGAGATGCGCTTGAA | For qPCR detection |
| SLA-DRα-F | GCCCTGAAGCCACTCTAA | For qPCR detection |
| SLA-DRα-R | GGAAAGCCAGCACAAGAA | For qPCR detection |
| SLA-DRβ-F | GAGGGCACGGTCTGAATC | For qPCR detection |
| SLA-DRβ-R | AGGGCGTCCTTTCTGATT | For qPCR detection |
a
Boldface indicates sequences that overlap with the corresponding NSPs, and italics indicate restriction enzyme cleavage sites.