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Detection of host restrictive factors stimulated by IFN-α and IFN-γ. A549 cells were treated with IFN-α (1,000 U/ml) or IFN-γ (1,000 U/ml) for 24 h and then harvested. Then, the mRNA levels of host restrictive factors were detected by RT-qPCR. GAPDH was used as a control (n = 3).
