FIG 3.

GBP5 restricts RSV replication in vitro. (A to C) HEK293T cells seeded in 6-well plates were transfected with 0.5 μg or 1.5 μg of plasmids expressing host factors. At 24 h posttransfection, the cells were infected with RSV at an MOI of 0.1 and then harvested at 48 h postinfection. (D to G) A549 cells stably expressing GBP5-HA and control cells were constructed and infected with RSV at an MOI of 0.1 for 24 or 48 h. (I to K) A549-sh GBP5 cells or control cells were treated or not with IFN-γ for 24 h, as indicated, and then infected with RSV at an MOI of 0.1 for 24 or 48 h. Viral proteins (A, E, and I) and viral mRNA (B, F, and J) levels in the cell lysates were detected by Western blotting and RT-qPCR, respectively. (C, G, and K) Virus titers in the supernatant were measured using the endpoint dilution titer method. For RT-qPCR, GAPDH was used as a control. (D and H) The viability of the stable cell lines constructed was detected using the CCK8 assay. For statistical analysis, n = 3.