Skip to main content
. 2020 Oct 14;94(21):e01407-20. doi: 10.1128/JVI.01407-20

FIG 7.

FIG 7

The RSV G protein downregulates GBP5 expression. (A) GBP5-HA was cotransfected with viral proteins coupled with an HA tag or VR1012 into HEK293T cells. At 48 h posttransfection, the cells were harvested and detected by immunoblotting. (B) GBP5-HA was cotransfected with gradient G-Flag into HEK293T cells. At 48 h posttransfection, the cells were harvested and detected by immunoblotting. (C) HEK293T cells cotransfected with GBP5-HA and G-Flag or empty vector (VR1012) for 24 h were treated with DMSO, 10 μM of MG132, or 10 nM Baf-A1 for an additional 10 h. The protein levels in the cells were then detected by immunoblotting. (D) GBP5-HA was cotransfected with G-Myc and Ub-K48-Flag or Ub-K63-Flag into HEK293T cells. At 24 h posttransfection, the cells treated with 10 μM MG132 for 10 h and then harvested and analyzed via an immunoprecipitation assay. The input and elution were detected by immunoblotting.