FIG 2.

IEC organoids are dually responsive to IFN-β and IFN-λ. (A) Representative images of IEC organoids. (B) IEC organoids were treated with the indicated concentrations of recombinant IFN-β or IFN-λ2 for the indicated times, and RNA was isolated for the quantitation of Isg15, Usp18, and Cxcl10 transcripts by qPCR. The dotted line indicates the limit of detection. (C) Transcript abundances of the indicated genes 4 h after IFN treatment across the range of IFN doses tested. Dashed lines indicate maximal responses for IFN-β or IFN-λ. (D) IEC organoids pretreated with PBS, IFN-β, or IFN-λ3 for 8 h were infected with rotavirus. Viral genomes were quantified at the indicated times postinfection. Data are from two (B and C) or three (D) experiments with duplicate treatment/infection wells; error bars show standard errors of the means (SEM). Statistical significance was determined by a t test (C) or one-way ANOVA (D). *, P < 0.05; **, P < 0.01.