FIG 8.

IFN-β-specific apoptosis ISGs are dependent on the canonical transcription factor STAT1. (A) Reanalysis of ChIP-seq data under GEO series accession number GSE115433 (52). Percentages of genes in common ISG and IFN-β-specific ISG sets that had significant ChIP-seq peaks for STAT1, STAT2, or IRF9 within 500 bp of the transcription start site are shown. Statistical significance was determined by a chi-square test of contingency tables of genes with or without peaks. *, P < 0.05; ****, P < 0.0001. (B and C) Quantitative PCR analysis of genes indicated on the x axis following treatment of WT or Stat1^−/− IEC organoids with 10 ng/ml IFN-β (B) or IFN-λ3 (C) for 4 h, normalized to PBS-treated controls. Data are combined from four experiments, with * indicating a P value of <0.05 by one-way ANOVA.