FIG 5.

The N-glycosylation site within EBV gL is likely the EphA2 binding site for EBV fusion. (A) CHO-K1 cells transfected with T7 luciferase plasmid, together with either vector plasmid (control), EBV gB with EBV gH/gL, or EBV gH/gL-N⁶⁹L/S⁷¹V, were overlaid with HEK293 cells transfected with control plasmid pcDNA 3.1, EphA2, or EphA4. EBV fusion with HEK293 cells transfected with pcDNA 3.1 was set to 100%. The fusion activity was normalized to EBV fusion with pcDNA 3.1-transfected cells, which was set to 100%. The data are means plus the SEM for three independent experiments. (B) Virus-free EBV or EBV gB843 fusion with WT HEK293T cells that overexpress EphA2 or EphA4. The bars represent the fusion activity, and the data are means plus the SEM for three independent experiments.