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. 2020 Oct 14;94(21):e01604-20. doi: 10.1128/JVI.01604-20

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Copyright © 2020 Madigan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — The intracellular calcium-chelating agent BAPTA-AM enhances AAV transduction. (a) AAV2 luciferase transduction of Scr and ATP2C1 KO Huh7 cells following treatment with BAPTA-AM (8 μM). (b and c) Luciferase reporter expression in HeLa cells pretreated with dimethyl sulfoxide (DMSO) or BAPTA-AM (20 μM) and infected with AAV1 luciferase (b) and AAV2 luciferase (c). (d) Transfection of HeLa cells treated with BAPTA-AM or DMSO control with pTR-CBA-luciferase. (e) HeLa cells pretreated with either DMSO control or BAPTA-AM and infected with AAV2-Luc at various vector doses. (f) Luciferase reporter expression in MB114 cells pretreated with DMSO or BAPTA-AM (20 μM) and infected with AAV2-luciferase. **, P < 0.01; ***, P < 0.005; ns, not significant.