FIG 2.

Introduction of ACE2 is required for infection of 293FT cells with SARS-COV-2 Spike. (A) Schematic of the HIV-1 Gaussia luciferase vector. (B) Transduction of 293FT cells transduced with different numbers of ACE2 genes with HIV-1-Gluc pseudoparticles pseudotyped with SARS-COV-2 Δ19 Spike or VSV-G. Transductions with VSV-G used 100 times less viral supernatant. Puro, vector with puromycin selection cassette; Blast, vector with blasticidin resistance cassette. Data are averages and standard errors for three independent experiments. Asterisks indicate significant differences (P < 0.05) by a paired Student t test; NS, no significant difference. (C) ACE2 surface expression of ACE2-expressing cell lines. Shown is an example representative of the results of three experiments.