Figure 5.

Diurnal differences in muscle protein phosphorylation. Volcano plot (A) presenting the log₂ fold change in phosphopeptide abundance between evening and morning conditions and the statistical significance determined by within-subject ANOVA (n = 9 participants). Phosphopeptides that were statistically different (p < 0.05) and had a false discovery rate <10% are annotated with their unique UniProt identifier and phosphorylation site; phosphopeptide-specific data (B) illustrating within-subject data connected by dashed lines and group data (median, quartile ranges) summarized by ‘box and whisker’ plots for the statistically significant (p < 0.05, FDR < 10%) phosphopeptides.