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Survival of larvae exposed to a dilution series of M. plutonius strain AF5. Larvae were inoculated in vitro (N = 24 for each concentration) and survival was recorded at Day 6. The control received diet and saline with no bacteria. We designated the 6.4 CFU/µL dilution as LD100, and the 0.64 CFU/µL dilution as LD50 for use as positive controls in the in vitro rescue assay.
