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. 2020 Sep 21;12(9):2693. doi: 10.3390/cancers12092693

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lobule types’ description and SFRP1 immunohistochemistry scores’ explanation. Column 1: Hematoxylin-eosin (H&E) staining of nontumoral tissue at different degrees of lobular involution (rows A and B: Partially involuted (1–74% TDLUs involuted) breast tissue obtained from premenopausal women without (row A) and with (row B) microcalcifications and rows C and D: Completely involuted (>74% TDLUs involuted) breast tissue obtained from premenopausal women with microcalcifications at light microscope ×5). Columns 2 and 3: Immunohistochemistry against SFRP1 (row A: Score 2; row B: Score 6, row C: Score 0; and row D: Score 1) at light microscope ×5 (column 2) and ×20 (column 3). TDLUs = terminal duct lobular units.