Fig. 2.

Factors affecting transient gusA expression of leaf disc explants of passion fruit using Agrobacterium strain LBA4404. a Effect of Agrobacterium cell density (OD600 values of 0.1, 0.25, 0.5 and 0.75); b Effect of infection time (10, 20, 30, 40 and 50 min); c Effect of co-cultivation period (1, 2, 3, 4 and 5 days); d Effect of concentration of acetosyringone (0, 50, 100, 150, 200 and 450 µM). Each experiment consisted of 30 leaf disc explants and experiments were done in triplicates. Treatments (mean ± SE) having the same letters are not significantly different at P > 0.05 using Tukey’s significant difference test