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. 2020 Oct 16;16:141. doi: 10.1186/s13007-020-00684-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Histochemical β-glucuronidase staining and PCR analysis of transformed leaf discs and transgenic plants. a Histochemical GUS staining of leaf discs after co-cultivation for 3 days; b Stable histochemical GUS staining of leaf segments of different transgenic plant lines and non-transformed control plant; and c PCR amplification of gusA gene fragment of DNA isolated from 6 transgenic plant lines. P = plasmid DNA, M = 1 kb molecular weight marker (Fisher Thermo scientific); NT = Non-transformed control plant; and L1–L6 = Transgenic plant lines 1 to 6, respectively