Table 1.

Experimental compositions of natural killer (NK) cell culture media.

Name	Composition
RPMI II	RPMI 1640 + 10% FBS + 1%Pen/strep + 50 ng/mL 4-1BBL + 10 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + 25 ng/mL rhIL-21
RPMI III	RPMI 1640 + 10% FBS + 1%Pen/strep + 50 ng/mL 4-1BBL + 10 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + 50 ng/mL rhIL-21
OpT without (w/o) Supplements	OpTmizer Basal Medium + 0% OpTmizer supplement + 5% hAB serum + 1% Pen/strep + 0.2 mM l-glutamine + 10 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + and 25 ng/mL rhIL-21
OpT w/o IL-15	OpTmizer Basal Medium + 2.6% OpTmizer supplement + 5% hAB serum + 1% Pen/strep + 0.2 mM l-glutamine + 0 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + and 25 ng/mL rhIL-21
OpT-RPMI 1640	RPMI 1640 + 2.6% OpTmizer supplement + 5% hAB serum + 1% Pen/strep + 0.2 mM l-glutamine + 10 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + and 25 ng/mL rhIL-21
OpT-FBS	OpTmizer Basal Medium + 2.6% OpTmizer supplement + 5% FBS + 1% Pen/strep + 0.2 mM l-glutamine + 10 ng/mL rhIL-15 + 500 IU/mL rhIL-2 + and 25 ng/mL rhIL-21
RPMI-hAB	RPMI 1640 + 10% hAB serum + 1%Pen/strep + 50 ng/mL 4-1BBL + 500 IU/mL rhIL-2 + 50 ng/mL rhIL-21

Deviations from the original RPMIf or OpTmizer™ recipes are underlined.