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. 2020 Aug 26;12(9):2417. doi: 10.3390/cancers12092417

Table 2.

Media compositions and their effect on the downregulation of TIM-3 on human NK cells in the presence of cancer cells across eight different media recipes. ‘+’ indicates the media component present in the recipe; ‘YES’ indicates that the recipe resulted in TIM-3 downregulation; ‘NO’ indicates that the media recipe did not result in TIM-3 downregulation.

Medium Component I II III IV V VI VII VIII
RPMI + + + +
OpT Basal + + + +
OpT Supp + + + +
AB Serum + + + + +
FBS + + +
IL-2 + + + + + + + +
IL-15 + + + + +
IL-21 + + + + + + + +
TIM-3 MFI decrease YES NO NO YES YES YES YES YES

RPMI: RPMI-1640; OpT Basal: OpTmizer™ basal medium; OpT Supp: OpTmizer™ supplement medium; AB Serum: Human AB Serum; FBS: fetal bovine serum.