Table 2.

Media compositions and their effect on the downregulation of TIM-3 on human NK cells in the presence of cancer cells across eight different media recipes. ‘+’ indicates the media component present in the recipe; ‘YES’ indicates that the recipe resulted in TIM-3 downregulation; ‘NO’ indicates that the media recipe did not result in TIM-3 downregulation.

Medium Component	I	II	III	IV	V	VI	VII	VIII
RPMI		+	+			+		+
OpT Basal	+			+	+		+
OpT Supp	+				+	+	+
AB Serum	+			+	+	+		+
FBS		+	+				+
IL-2	+	+	+	+	+	+	+	+
IL-15	+		+	+		+	+
IL-21	+	+	+	+	+	+	+	+
TIM-3 MFI decrease	YES	NO	NO	YES	YES	YES	YES	YES

RPMI: RPMI-1640; OpT Basal: OpTmizer™ basal medium; OpT Supp: OpTmizer™ supplement medium; AB Serum: Human AB Serum; FBS: fetal bovine serum.