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. 2020 Aug 19;8(3):458. doi: 10.3390/vaccines8030458

Figure 1.

Figure 1

Schematic diagram and expression of chimeric HA and NA proteins. (A) Schematic diagram of chimeric HA and NA proteins. Domains derived from A/Puerto Rico/8/34 (H1N1) virus (designated as PR8) are shown as light blue boxes and that of A/California/07/09 (H1N1) virus (designated as Cal) as red boxes. Designations of chimeric HA and NA are shown to the right of constructs. SP—signal peptide; TM—transmembrane; CT—cytoplasmic tail. The amino acid numbers are shown in diagrams. (B) Expression of chimeric HA and NA proteins. MDCK cells were cotransfected with an HA or NA expression plasmid and an EGFP expression plasmid. Protein expression was analyzed by Western blotting with anti-H1 HA rabbit Ab, anti-PR8 HA mAb, anti-Cal HA mAb, and anti-NA sheep Ab. The blots were also probed with anti-GFP and anti-actin mAbs. Representative blots are shown. The band intensities were semi-quantified with ImageJ software.