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. 2020 Aug 9;8(3):448. doi: 10.3390/vaccines8030448

Figure 4.

Figure 4

Electrophoretic analysis of RT-PCR products in 1% agarose gel. 1, 2, 3, RT-PCR products were obtained using cDNAs from 293T cells transfected with the target plasmids: 1, p-AgH1; 2, p-AgH3; 3, p-AgM2; 1RNA, 2RNA, and 3RNA, PCR reaction in these probes was performed without reverse transcription of RNAs isolated from transfected 293T cells (this was done to check both primers specificity and the lack of plasmids admixtures); M, M12 oligonucleotide size markers produced by SibEnzyme (DNA fragments 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 750, 500, and 250 bp).