Figure 1.

Lentiviral vectors and modified clonal neural stem cell lines. Neural stem cells were transduced with a lentiviral vector encoding glial cell line-derived neurotrophic factor (GDNF) and enhanced green fluorescent protein (eGFP) (a) or ciliary neurotrophic factor (CNTF) and Venus (b). Cells for control experiments were transduced with a vector encoding Venus only (c). Immunocytochemical analysis of a culture composed of a 1:1 mixture of a GDNF- and a CNTF-overexpressing neural stem cell line revealed that cells co-expressed either eGFP and GDNF (d,e) or Venus and CNTF (d,f). Cells for control experiments expressed Venus (h) but neither GDNF (i) nor CNTF (j). The CNTF signal in (g,k) (overlays of (e,f) and (i,j), respectively) is shown in green. Scale bar: 50 µm.