FIG 2.
Genome-wide occupancy of the transcription factor Nsi1 with ChEC-seq. (A) Temporal analysis of Nsi1 binding events. Venn diagram showing the overlap of Nsi1 binding events at three distinct MNase activation times (5, 20, and 60 min). (B) Motif scores for Nsi1-bound promoters at 5, 20, and 60 min after MNase activation. The motif logos were generated using MEME-ChIP software on the 1,000 high-scoring peaks. (C to F) Snapshots of genomic regions showing the ChEC-seq signal for Nsi1MNase and the free MNase strains at 5, 20, and 60 min after MNase activation. The positions of the Nsi1 motifs are indicated by the dashed lines. Nsi1 occupies the promoter of MLC1 (C), AIM7 (D), and ASM4 (E), in addition to many sites within the rDNA locus (F).