FIG 6.

Antifungal drugs differentially modulate MAPK responses in C. auris. (A) Caspofungin treatment modulates MAPK signaling pathways in C. auris. Logarithmically growing Candida auris cultures were treated with 100 ng/ml caspofungin for 15 min and washed once with ice-cold water, and whole-cell extracts were prepared for immunoblotting as described in the legend to Fig. 5A. Densitometry was performed using Image Studio software (Li-Cor). Data are expressed as fold change normalized to the PSTAIR (Cdc28) loading control; extracts from untreated control strains were set to 1. Results are from two independent biological samples (±SD; *, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005; ****, P < 0.0001). Striped columns indicate the drug (CAS) treatment. (B) Amphotericin B treatment modulates MAPK signaling pathways in C. auris. Logarithmically growing Candida auris cultures were treated with 500 ng/ml amphotericin B for 15 min and washed once with ice-cold water, and whole-cell extracts for immunoblotting were prepared as described above and subjected to immunoblotting as described for panel A. Striped columns indicate drug (AMB) treatment conditions.