Figure 4.

XF conditions enhance the expression of immunoregulatory factors in WJ-MSCs. WJ-MSCs were cultured in FBS and XF conditions were treated with or without IFN-γ (10 ng/mL) and TNF-α (10 ng/mL) for 24 h. (A) Gene expression of the immunoregulatory and inflammatory factors was determined by qRT-PCR (* p < 0.05, ** p < 0.01). (B) After IFN-γ and TNF-α treatment for 24 h, IDO, TGF-β1, NOS2, and TSG-6 expression were measured by Western blot analysis. The results are representative of independent experiments performed at least three times.