. 2020 Sep 3;11(3):62. doi: 10.3390/jfb11030062

Table 1.

Real-time reverse transcriptase polymer chain reaction (RT-PCR) details, including gene, primer sequences, operational conditions, and product size.

Gene	Primers Sequence	Conditions	Product Size (bp)
IL-1α	5′-CATGTCAAATTTCACTGCTTCATCC-3′	5 s at 95 °C, 8 s at 55 °C, 17 s at 72 °C for 45 cycles	421
IL-1α	5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	5 s at 95 °C, 8 s at 55 °C, 17 s at 72 °C for 45 cycles	421
IL-1β	5′-GCATCCAGCTACGAATCTCC-3′	5 s at 95 °C, 14 s at 58 °C, 28 s at 72 °C for 40 cycles	708
IL-1β	5′-CCACATTCAGCACAGGACTC-3′	5 s at 95 °C, 14 s at 58 °C, 28 s at 72 °C for 40 cycles	708
IL-6	5′-ATGAACTCCTTCTCCACAAGCGC-3′	5 s at 95 °C, 13 s at 56 °C, 25 at 72 °C for 40 cycles	628
IL-6	5′-GAAGAGCCCTCAGGCTGGACTG-3′	5 s at 95 °C, 13 s at 56 °C, 25 at 72 °C for 40 cycles	628
IL-8	5′-ATGACTTCCAAGCTGGCCGTG-3′	5 s at 94 °C, 6 s at 55 °C, 12 s at 72 °C for 40 cycles	297
IL-8	5′-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′	5 s at 94 °C, 6 s at 55 °C, 12 s at 72 °C for 40 cycles	297
TNF-α	5′-CAGAGGGAAGAGTTCCCCAG-3′	5 s at 95 °C, 6 s at 57 °C, 13 s at 72 °C for 40 cycles	324
TNF-α	5′-CCTTGGTCTGGTAGGAGACG-3′	5 s at 95 °C, 6 s at 57 °C, 13 s at 72 °C for 40 cycles	324
TGF-β	5′-CCGACTACTACGCCAAGGAGGTCAC-3′	5 s at 94 °C, 9 s at 60 °C, 18 s at 72 °C for 40 cycles	439
TGF-β	5′-AGGCCGGTTCATGCCATGAATGGTG-3′	5 s at 94 °C, 9 s at 60 °C, 18 s at 72 °C for 40 cycles	439
HBD-2	5′-GGATCCATGGGTATAGGCGATCCTGTTA-3′	5 s at 94 °C, 6 s at 63 °C, 10 s at 72 °C for 50 cycles	198
HBD-2	5′-AAGCTTCTCTGATGAGGGAGCCCTTTCT-3′	5 s at 94 °C, 6 s at 63 °C, 10 s at 72 °C for 50 cycles	198