Figure 4.

Ybx1 depletion results in developmental defects due to cytokinesis failure in zebrafish embryos. (a) Examples of zebrafish embryos at 4.3, 4.7, 5.7, 6 and 48 h post fertilization treated 4 ng of either control morpholino (MO_Ctrl, top panel) or a morpholino targeting ybx1 (MO_ybx1, bottom panel). Images were acquired using 10× objective. (b) Examples of zebrafish embryonic cells that have been treated with 4 ng of MO_Ctrl (top panel) or MO_ybx1 (bottom panel) 5.7–6 h post-fertilization undergoing cytokinesis, immunostained with antibodies to Ybx1 (green) and α-tubulin (cyan). Images were acquired using a 60× oil-immersion objective.