Figure 2.

Representative images of liver cross-sections stained with hematoxylin and eosin (H&E) or Sirius Red (A) and quantitative analysis (B–F) from Ldlr−/−.Leiden mice fed a healthy chow diet or fed a high-fat diet (HFD) containing lard or a fast food diet (FFD) containing milk fat for 28 weeks. Macrovesicular (B) and microvesicular (C) steatosis as a percentage of total liver area, inflammation as number of inflammatory aggregates per mm² microscopic field (D), and hepatic fibrosis were analyzed as percentage Sirius Red of surface area (E) or biochemically analyzed hepatic collagen/mg protein (F). Values represent mean ± SEM for ≥8 mice per group. ** p < 0.01 and *** p < 0.001 vs. chow; # p < 0.05, ## p < 0.01 and ### p < 0.001 FFD vs. HFD.