Table 3.
Preparation and delivery of FMT material.
| Author and Year | Donor(s) | Fresh/Frozen | FMT Preparation | Concomitant Treatment | Pre-Treatment with Antibiotics | Single/Multi-donor | Bowel Cleansing |
|---|---|---|---|---|---|---|---|
| Fang et al., 2016 | Unrelated donor | Fresh | Stool mixed with sterile saline and diluted to 250 mL | The patient was off his usual antibiotics during the entire follow-up period but continued antidiarrheal medication. | Antibiotics stopped 48 h before FMT | Single | No |
| Herfarth et al., 2019 | Unrelated donor with high butyrate production | Frozen | Stool bank provided FMT for endoscopic administration, FMT capsules and matching placebos | NA | Antibiotics stopped 24 h before FMT | Single | NA |
| Kousgaard et al., 2020 | Unrelated | Frozen | 20 g stool mixed with 100 mL sterile water, blended and filtered, 10% glycerol | Concurrent therapies were permitted if stable with exception of antibiotics, probiotics, and biologic treatment. | Antibiotics stopped 7 days before FMT | Multi-donor (5 donors) | No |
| Landy et al., 2015 | Relative/partner (6) or anonymous (2) | Fresh (less than 6 h prior to FMT) | 30 g of stool was homogenized with a household blender in 50 mL of 0.9% saline and filtered through sterile gauze to produce a fecal-saline solution | Night before the procedure the recipient was treated with a proton pump inhibitor (Omeprazole 20 mg). | NA | Single | NA |
| Nishida et al., 2019 | Second degree relatives | Fresh (less than 4 h prior to FMT) | 150–200 g donor stool mixed with 350–500 mL sterile saline and filtered through gaze | NA | NA | Single | Polyethylene glycol solution |
| Schmid et al., 2017 | Patient’s son | Fresh (less than 6 h prior to FMT) | A fresh stool sample diluted with 500 mL saline and filtered through gaze | NA | NA | Single | Enemas |
| Selvig et al., 2020 | Unrelated | Frozen | 25 g of stool mixed with saline to a 250 mL fecal suspension | Accepted with antibiotics as exception. Three patients continued biologic treatment. | 7/18 patients received 5 days of 550 mg Rifaximin beginning 8 days prior to treatment | Single (from 11 different donors) | Magnesium citrate a day before FMT and phosphate enema at day of FMT |
| Stallmach et al., 2016 | Unrelated | Fresh (first FMT) and frozen | 150 g stool mixed with 400 mL saline. Then filtered and separated in two. The first immediately delivered to the patient and the second stored at −80 °C | NA | NA | Single (from 2 different donors) | No |
| Steube et al., 2017 | Unrelated | NA | NA | NA | NA | Single (from 3 different donors) | NA |
NA, not available; FMT, Fecal Microbiota Transplantation.