Figure 2.

miR-4721 Promotes NPC Proliferation In Vitro and In Vivo

(A and C) MTT assays (A) and EdU incorporation assays (C) were conducted after transfection with miR-4721 mimics or inhibitor. Student’s t test. Mean ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars, 200 μm. (B) Colony formation assays were performed after transfection with lentiviral particles carrying the miR-4721 precursor or negative control. Student’s t test. Mean ± SD. ∗∗∗p < 0.001. (D) Cell cycle of HONE1 and SUNE1 cells transfected with miR-4721 inhibitors with/without mimics (n = 3). Student’s t test. Mean ± SD. ∗p < 0.05, ∗∗p < 0.01. (E) Xenograft tumors collected on day 15 after subcutaneous implantation of SUNE1-NC, SUNE1-miR-4721, HONE1-NC, and HONE1-miR-4721 cells on nude mice. Tumor volume and tumor weight were measured on day 15 (n = 5), Student’s t test. Mean ± SD. ∗∗p < 0.01, ∗p < 0.05. (F) Representative H&E staining as well as PCNA and Ki-67 IHC of primary tumor tissues are shown. Original magnification, ×200; scale bars, 30 μm. Mean ± SD (n = 3). ∗∗p < 0.01.