Figure 4.

miR-7-5p Regulates HPASMC Osteogenic Transition

(A) Representative images from sections stained with ARS. (B) Runx2 expression was detected by immunofluorescence staining. (C) ALP activity in the cells transfected with NC or miR-7-5p mimics was measured. (D) The calcium content in calcified HPASMCs was assayed. (E) Western blot analysis of osteogenic markers in HPASMCs exposed to Hyp with or without agomir. (F) Calcium deposition was determined after cotransfecting cells with siCDR1as and antagomir of miR-7-5p. (G) The expression level of Runx2 in HPASMCs of the indicated groups was determined by immunofluorescence assay. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. All tests were performed at least three times, and the values are presented as the mean ± SEM.