Figure 6.

Effects of miR-18a on Cell Invasion and Proliferation in HTR8/SVneo Cells

(A and B) Quantitative real-time PCR results to reveal the miR-18a expression in HTR8/SVneo cells transfected with miR-18a mimics and scramble control (NC) (A) and miR-18a inhibitor or scramble inhibitor (inhibitor-NC) (B). The level of miR-18a was adjusted by the value of U6, and the relative value is presented as the mean ± SD, based on 3 independent experiments. ∗p < 0.05. (C and D) Transwell insert assay to examine cell invasiveness in HTR8/SVneo cells transfected with NC and miR-18a (C) and inhibitor NC or miR-18a inhibitor (D). The invasion index is presented as mean ± SD, based on 3 independent experiments. ∗p < 0.05. (E) The effect of miR-18a on the cell growth of HTR8/SVneo cells. The data are presented as the mean ± SD, according to the results of three independently repeated experiments. (F) The effect of miR-18a on the cell cycle of HTR8/SVneo cells. The data are presented as the mean ± SD, according to the results of three independently repeated experiments. (G) Smad2(FL) rescued the effect of miR-18a on cell invasion in HTR8/SVneo cells. The cells were transfected with miR-18a and Smad2 alone or in combination with scramble siRNA (NC) or pcDNA4 vector (pcDNA4) as corresponding NC. The Transwell insert assay was performed to monitor cell invasiveness. The bar chart represents statistical analysis based on 3 independently repeated experiments. Data are presented as mean ± SD. ∗p < 0.05.