Table 2.

Sequences of the Primers Used for Plasmid Construction or Real-Time PCR

Product	Primer Sequence (5′ to 3′)
GAPDH real-time PCR	F: AAGGTCATCCCTGAGCTGAAC
GAPDH real-time PCR	R: ACGCCTGCTTCACCACCTTCT
pri-miR-18a real-time PCR	F: CAGTAAAGGTAAGGAGAGCTCA ATCTG
pri-miR-18a real-time PCR	R: CATACAACCACTAAGCTAAAG AATAATCTGA
miR-18a real-time PCR	F: TAAGGTGCATCTAGTGCAGATAG
U6 real-time PCR	F: CGCAAGGATGACACGCAAATTC
Smad2(FL) real-time PCR	F: TGGGCAGGAAGAAAAGTGGT
Smad2(FL) real-time PCR	R: GGCCTGTTGTATCCCACTGA
Smad2(Δexon3) real-time PCR	F: GAGCAGAATGGGCAGGAAGA
Smad2(Δexon3) real-time PCR	R: ACGACCATCAAGAGACCTTGG
BD-WT	F: GGACTAGTTCATAGCATTGTG TGTGGTCC
BD-WT	R: CCCAAGCTTTAAACAGCACAA TACTGTGA
BD-MUT	F: GGTCTCATCAATTAAAGCAGC TTGTGGAATCTGTTCCT
BD-MUT	R: AGGAAACAGATTCCACAAGC TGCTTTAATTGATGAGACC
Smad2(FL)-expressing plasmid	F: GGAATTCAAGAGGCTGTTTTC CTAGCGT
Smad2(FL)-expressing plasmid	R: CCGCTCGAGCATTAAGTCTTT TCATGGGAC

F, forward; R, reverse.