Table 1.

DNA sequence of broken beacon probes and location of attached modifications. TAMRA is excited at a wavelength of 557 nm and emits at a wavelength of 583 nm. BHQ2 maximally quenches at 579 nm.

DNA Sequence 5′ – 3′	Modifications
ttcacatttcatcgacggacaacg	5′ – TAMRA
cgatgaaatgtgaa	3′ – BHQ2