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. 2020 Oct 15;17:303. doi: 10.1186/s12974-020-01989-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Experimental design, cytoprotective and antioxidant effects of glycine in d-galactose-treated in HT22 cells. a Experimental design of experimental work for the drug treatment in C57BL/6 mice and behavioral analysis. b–d Results of MTT (cell viability) assay and their relative histogram in neuronal HT22 cells. Number of experiment (N = 3). e, f Representative histograms of reactive oxygen species (ROS measured as DCF level) and lipid peroxidation (LPO measured MDA level) assays of Gly against D-gal in HT22 cells. The data are shown as the mean ± SEM of triplicate wells for in vitro experiments are representative of three independent experiments. Significance: Asterisk (*) sign indicated significant difference from the normal saline-treated control group; hash (#) sign indicated significant difference from d-gal-treated group. Significance: *P ≤ 0.05, **P ≤ 0.01; ***P ≤ 0.001; #P ≤ 0.05, ##P ≤ 0.01. n.s no significance