Fig. 4.
Effect of Bacillus amyloliquefaciens CECT 5490 (1.0 × 106 CFU/g of feed) (BA) on intestinal tight junction related gene expression in broilers at day 16. (a-d) Effect of NE challenge and BA supplementation on occludin (OCLD), tight junction proteins-1 (TJP1), mucin-2 (MUC2), and claudin-1 (CLDN1) expression level; (e-d) Effect of crude protein (CP) level and BA supplementation on OCLD, TJP1, MUC2, and CLDN1 expression level. Non: non-challenged; NE: NE challenged; SCP: Standard protein diet; RCP: Reduced protein diet (2% lower than standard diets). Genes used as reference genes were HMBS and YWHAZ. Error bars show SEM for 8 replicates for each treatment